Biotechnological innovation meets horticulture as researchers unveil a cutting-edge genome editing protocol for grapes. This novel technique, integrating protoplast culture with CRISPR-Cas9, ...

Protoplast culture and plant regeneration of an important medicinal plant Tylophora indica were achieved through callus regeneration. Protoplasts were isolated from leaf mesophyll cells and cultured ...

This is a preview. Log in through your library . Abstract Protoplasts have been isolated without the application of wall degrading enzymes from three large brown algal species: Macrocystis ...

Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by sequencing, ...