We understand that gaining publication-quality results straight away is not the norm when performing western blot. This 32-page handy guide provides tips and tricks to obtain high-quality, publishable ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...