This 5-day training is designed to provide a solid understanding of specific topics through presentation and laboratory work.Participants will gain significant experience in the performance of ...

How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

Digital PCR (dPCR) is a specialized approach to nucleic acid detection and quantification that is not reliant on standard curves. Instead, the quantity or numbers of molecules present in a reaction is ...

PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...

The perceived ease of use of real-time quantitative PCR (qPCR) and reverse transcription PCR (RT-qPCR) technology has revolutionized life science research. Its effectiveness at amplification and ...

Respiratory syncytial virus (RSV) was detected easiest when using reverse transcription polymerase chain reaction (RT-PCR) tests in a pediatric population. Reverse transcription polymerase chain ...

With the increasing importance of generating large-scale data to drive new pharmaceutical and biotech discoveries, many scientists are feeling pressure to speed up data collection. One increasingly ...

In a recent study published in Lancet Microbe, researchers compare the potential of real-time reverse transcriptase-polymerase chain reaction (RT-PCR) genotyping assays with whole-genome sequencing ...

This is a preview. Log in through your library . Abstract Real-time polymerase chain reaction (real-time PCR), also known as quantitative PCR, is used to determine relative gene expression or to ...

During the early stages of the coronavirus disease 2019 (COVID-19), rapid testing was imperative for public health policymakers and health care workers in order to determine where and what measures ...