Accurate assembly of rRNA-depleted (total) RNA sequencing (RNA-seq) remains challenging because existing methods often conflate incomplete, nascent RNA with fully processed mature isoforms, leading to ...

Researchers from Children's Hospital of Philadelphia (CHOP) developed a new RNA sequencing strategy that can reveal how genetic variants disrupt gene function and improve the diagnosis of rare ...

Single-cell RNA-sequencing (scRNA-seq) is an increasingly popular method for investigating properties of heterogeneous tissues. Importantly, it allows the assessment of tumor heterogeneity and the ...

RNA-seq succeeded across all tumor types and tissue formats, including degraded FFPE samples. Researchers conclude that targeted RNA-seq can serve as a stand-alone molecular diagnostic with high yield ...

A new single-cell technology is giving scientists their clearest view yet of immune cell behavior—capturing not just genetic intent, but real-time activity. By measuring RNA and proteins ...

Morgan Hiebert (left) studies drug-resistant tuberculosis within the National Reference Centre for Mycobacteriology (Winnipeg, Manitoba) led by Hafid Soualhine, situated in the National Microbiology ...

While bulk sequencing quickly became the preferred method, scRNA-seq has increased in popularity over the last decade. In 2015, just 6 % of RNA-seq papers used single-cell methodologies. By 2025, the ...

A comprehensive review article titled “Bioinformatics perspectives on transcriptomics: A comprehensive review of bulk and single-cell RNA sequencing analyses,” published in Quantitative Biology, ...

Understand the stages of a standard RNA-seq bioinformatics workflow, including the role of QC, alignment, gene expression quantification, and specialized application analyses Identify key QC metrics ...

Inside every cell, thousands of molecular signals collide, overlap, and compensate, obscuring the true drivers of gene expression. Scientists have now developed a way to silence that cellular noise, ...

SINGAPORE – Researchers from A*STAR Genome Institute of Singapore (A*STAR GIS) have developed a new method, called ‘sm-PORE-cupine’, to study individual RNA molecules and reveal how their structures ...

Bacteria aren’t just mindless microbes. Research by scientists at the Hebrew University of Jerusalem has revealed that single bacterial cells can carry a “memory” of their past environments—passing it ...