High-throughput screening (HTS) is crucial to generate hits and leads in drug discovery efforts. Most high-throughput biochemical assays are based on optical detection or radioactivity with high ...

Traditional 2D monolayer cell cultures often fail to accuratelyreplicate the complex cellular microenvironment found in living organisms. In contrast, 3D cell-based assays allow cells to grow into ...

Aims In this study, we analyze the challenges involved in detecting novel neuraminidase inhibitors (NAIs) and offer strategies to overcome them with complementary bioassays. Materials & Methods We ...

In drug development projects, evaluating drug-target residence times can enhance drug efficacy, widen the therapeutic window, and reduce the likelihood of prematurely advancing candidate compounds ...

Whether They Are Used In Drug Screens or Precision Medicine, Cell-Based Assays Are All About Context Cells are miniature laboratories that allow scientists to study key biological processes. As a ...

Kirsten rat sarcoma viral oncogene homologue (KRAS) is the most frequently mutated oncogene, present in approximately 25% of tumors. Numerous studies on KRAS in the initiation and advancement of ...

Researchers engaged in drug discovery and development seek strategies for understanding the dynamics of drug-target interactions. Measuring the drug-target residence time—the duration of time that a ...

A mutant DJ-1 gene causes recessive Parkinson's disease, but the molecular mechanism of this process has not been well studied. To understand how DJ-1 hydrolyzes cyclic 3-phosphoglyceric anhydride—a ...