Epitope tagging is a technique that employs genetic engineering to fuse a known epitope, called an affinity tag, to either the C or N terminus of a recombinant protein to facilitate affinity ...

Creating analytical tools that support the advancement of knowledge can often come about through the integration of existing information and techniques, theoretical and practical, directed toward a ...

Protein purification can be a challenging process. Since proteins are expressed in biological systems such as cells, the protein must be purified from a complex mixture. Generic column-based ...

Affinity chromatography theoretically does just that—a ligand that specifically interacts with the target protein is immobilized on a chromatography matrix; the target protein binds to the column, and ...

Self-removing affinity tags have the potential to greatly accelerate the development of new therapeutics, while providing a potentially enabling manufacturing platform for non-mAb therapeutic proteins ...

Although affinity tag methods for protein purification have greatly accelerated research in laboratories throughout the world, the presence of the tag on the purified target can modify the target and ...

Protein yield and ease of purification are two important criteria in choosing a system for the expression/ purification of recombinant proteins. The IMPACT (Intein Mediated Purification with an ...

The present purification protocol applies to target proteins that are fused to a double tag, such as NusA–His 6, through a linker that includes a protease-recognition sequence. It involves two steps ...

The emergence of carbapenemase-producing multi-drug resistant Enterobacteriaceae poses a dramatic, world-wide health risk. Limited treatment options and a lack of easy-to-use methods for the detection ...